Premium
GABA‐like immunoreactivity in the cat retina: Light microscopy
Author(s) -
Wässle H.,
Chun M. H.
Publication year - 1989
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.902790105
Subject(s) - inner plexiform layer , muscimol , retina , biology , axon , microbiology and biotechnology , neuroscience , anatomy , gabaa receptor , biochemistry , receptor
Semithin sections of the cat retina were stained with antibodies against GABA conjugated to bovine serum albumin with glutaraldehyde. Labelled cells were visualized by means of the peroxidase‐antiperoxidase method. In the outer plexiform layer both A‐ and B‐type horizontal cells and the B‐type axon terminal system expressed GABA‐like immunoreactivity. Approximately 25–30% of all amacrine cells and the whole inner plexiform layer were heavily labelled. Two types of putative GABA‐ergic interplexiform cells could be distinguished. One of them also expressed tyrosine‐hydroxylase‐like immunoreactivity. A few bipolar cells were also GABA‐immunolabelled. GABA‐like immunoreactivity and 3 H‐muscimol uptake were colocalized in 90% of the amacrine cells labelled. However, horizontal cells did not accumulate 3 H‐muscimol.