Premium
Binding pattern of alpha‐bungarotoxin on horizontal cells of a marine teleost retina
Author(s) -
Deplano Stefania
Publication year - 1988
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.902770407
Subject(s) - inner plexiform layer , retina , biology , bungarotoxin , nicotinic agonist , biophysics , microbiology and biotechnology , acetylcholine receptor , receptor , outer plexiform layer , anatomy , inner nuclear layer , neuroscience , alpha (finance) , biochemistry , medicine , construct validity , nursing , patient satisfaction
A conjugate of alpha‐bungarotoxin and a fluorescent marker (fluorescein isothiocyanate) has been used to localize “nicotinic” acetylcholine receptors on neurons in the outer plexiform layer of marine teleost retina. Toxin binding was confined to bipolar cell dendrites and to intermediate horizontal cells. The arrangement of labeled horizontal cells appears irregular inthe whole retina, with a peak density in the ventral and dorsal quandrants. Alpha‐bungarotoxin receptors on horizontal cells differ from those on bipolar cells and from those on dendrites in the inner plexiform layer in their sensitivity to agonists and antagonists such as d‐tubocurarine and nicotine. They consistute a different type of “nicotinic” receptor that probably has a different function.