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Immunohistochemical studies of cholecystokininlike peptides and their relation to 5‐HT, CGRP, and bombesin immunoreactivities in the brainstem and spinal cord of lampreys
Author(s) -
Brodin Lennart,
Buchanan James T.,
Hökfelt Tomas,
Grillner Sten,
Rehfeld Jens F.,
Frey Peter,
Verhofstad Albert A. J.,
Dockray Graham J.,
Walsh John H.
Publication year - 1988
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.902710103
Subject(s) - calcitonin gene related peptide , bombesin , spinal cord , biology , brainstem , reticular formation , antiserum , medicine , cholecystokinin , neuropeptide , reticular connective tissue , endocrinology , central nervous system , anatomy , neuroscience , antibody , receptor , biochemistry , immunology
The distribution of cholecystokinin (CCK)‐like immunoreactivity in the brainstem and spinal cord of lampreys was studied by using CCK antisera with different properties. In the spinal cord, three separate systems reacted with CCK antisera: (1) A ventral and lateral fiber system descending from a group of neurons in the posterior reticular nucleus of the rhombencephalon was labeled by both a C‐terminal‐directed CCK antiserum and a monoclonal CCK antibody. (2) A dorsal root–dorsal column system of fibers originating from cell bodies in the dorsal root ganglia was labeled only by the C‐terminal CCK antiserum. This CCK immunoreactivity could be abolished by preabsorption with calcitonin‐gene‐related peptide (CGRP), suggesting that it was due to cross‐reactivity with a CGRP‐like peptide. This system also contained 5‐hydroxytryptamine (5‐HT)‐, bombesin‐, and CGRP‐like immunoreactivities. (3) An intraspinal system of 5‐HT neurons was labeled with an antiserum to the midportion of CCK‐33 but not by the other CCK antisera. The CCK labeling of this system was difficult to reduce by preabsorption with CCK peptide and thus appeared to be nonspecific. Groups of cell bodies in the middle reticular nucleus of the rhombencephalon, the reticular nucleus of the mesencephalon, and the hypothalamus were labeled by both the C‐terminal and the monoclonal CCK antisera. The gut contained two types of CCK‐like immunoreactivity, one of which appeared to be due to cross‐reactivity with CGRP. A biochemical analysis showed that the content of CCK was low in the spinal cord compared to the brain, and these results agreed with the immunohistochemical findings.