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Distribution of specific intermediate‐filament proteins in the goldfish retina
Author(s) -
Jones Paul S.,
Schechter Nisson
Publication year - 1987
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.902660109
Subject(s) - neurofilament , biology , intermediate filament , retina , vimentin , cytoskeleton , intermediate filament protein , microbiology and biotechnology , antiserum , retinal , eye proteins , ganglion cell layer , polyclonal antibodies , protein filament , immunohistochemistry , antibody , biochemistry , cell , immunology , neuroscience
The intermediate‐filament proteins expressed in the goldfish retina were investigated by immunohistochemistry and by immunoblotting. Polyclonal antibodies that previously had been raised against the goldfish optic nerve neurofilament (ON 1 and ON 2 ) and glial filament (ON 3 and ON 4 ) proteins were used in this study. Anti‐ON 1 /ON 2 antiserum reacted on a retinal immunoblot with two proteins having molecular weights and isoelectric points corresponding to those of ON 1 and ON 2 . Histologically, the most pronounced anti‐ON 1 /ON 2 reactivity was observed in the ganglion cell layer of the goldfish retina. The anti‐ON 3 /ON 4 antiserum reacted with a single protein on a retinal immunoblot. This protein had a molecular weight and isoelectric point which corresponded to the goldfish optic nerve glial filament proteins. This anti‐serum labeled horizontal cells in retina sections. Three previously unidentified goldfish visual‐pathway intermediate‐filament proteins sharing a molecular weight of 60K were observed on two‐dimensional gels of retinal cytoskeletal proteins and on retinal immunoblots which were probed with a monoclonal antibody which recognizes an epitope common to all intermediate Filament proteins. The possible existence of homologs of mammalian GFAP and vimentin in the goldfish retina was also explored. Antibodies directed against mammalian GFAP and vimentin labeled the Müller fibers and the cone horizontal cells, respectively. However, immunoblot analysis and a comparison of the two‐dimensional gel electrophoresis patterns of goldfish retinal and rat spinal cord cytoskeletal proteins demonstrated a lack of goldfish proteins identical to the mammalian intermediate‐filament proteins.