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Mapping of neuropeptide Y‐like immunoreactivity in the feline hypothalamus and hypophysis
Author(s) -
Leger Lucienne,
Charnay Yves,
Danger JeanMichel,
Vaudry Hubert,
Pelletier Georges,
Dubois PaulMarie,
Jouvet Michel
Publication year - 1987
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.902550211
Subject(s) - median eminence , hypothalamus , arcuate nucleus , pituitary stalk , neuropeptide y receptor , biology , cell bodies , nucleus , neuropeptide , anatomy , population , endocrinology , medicine , suprachiasmatic nucleus , pituitary gland , central nervous system , neuroscience , hormone , biochemistry , receptor , environmental health
The distribution of neuropeptide Y (NPY) in the cat hypothalamus and hypophysis was studied with the indirect immunofluorescence technique of Coons and co‐workers (Coons, Leduc, and Connolly: J. Exp. Med. 102 :49–60, 1955), which provided a detailed map of NPY‐like immunoreactive neurons. The immunolabelling was detected in cell bodies, fibers, and terminallike structures widely distributed throughout the whole hypothalamus. A large population of medium‐sized NPY‐like immunoreactive cell bodies was localized in the area of arcuate nucleus. The number of immunoreactive cell bodies visualized was dramatically increased after intracerebroventricular injections of colchicine. Numerous immunolabelled cell bodies were also visible in the median eminence and scattered in the lateral hypothalamic area. Dense plexuses of NPY‐immunoreactive fibers were observed in the arcuate nucleus, internal layer of median eminence, periventricular zone, and paraventricular nucleus. Other regions of hypothalamus displaying numerous NPY‐like immunoreactive fibers included dorsal and ventrolateral hypothalamic areas. In contrast, certain hypothalamic areas were almost devoid of NPY‐like immunoreactive fibers—namely, the mamillary bodies and suprachiasmatic nucleus. Finally, in neurohypophysis, bright immunofluorescent fibers were observed along the pituitary stalk and penetrating the neural lobe. These results suggest the widespread distribution of the NPY‐containing neuronal systems in the cat hypothalamus and hypophysis.