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Comparative distribution of 3 H‐GABA uptake and GAD immunoreactivity in goldfish retinal amacrine cells: A double‐label analysis
Author(s) -
Yazulla Stephen,
Studholme Keith,
Wu JangYen
Publication year - 1986
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.902440203
Subject(s) - inner plexiform layer , inner nuclear layer , colocalization , biology , gabaergic , ganglion cell layer , retina , amacrine cell , glutamate decarboxylase , population , ganglion , microbiology and biotechnology , neuroscience , inhibitory postsynaptic potential , biochemistry , demography , sociology , enzyme
The comparative distribution of 3 H‐GABA uptake and glutamic acid decarboxylase immunoreactivity (GAD‐ER) in amacrine cells of goldfish retina was studied simultaneously by using a combined autoradiographic/immunocytochemical technique in order to determine the degree of colocalization of these two markers of GABAergic neurons; 3 H‐GABA was taken up most intensely by large pyriform Ab amacrine cells (3% of inner nuclear layer (INL) somata), and less intensely by smaller, polyform amacrine cells (12% of INL somata), cell bodies in the ganglion cell layer (one‐half as common as Ab cells), and cell bodies in the inner plexiform layer (very rare). GAD‐IR was observed in 25% of amacrine cells in the INL, the vast majority of which were polyform in shape, and cell bodies in the ganglion cell layer. Twice as many cells were labeled for GAD‐IR as for 3 H‐GABA uptake. Of the cells that took up 3 H‐GABA, colocalization of 3 H‐GABA uptake and GAD‐IR was observed in 90% of the polyform amacrine cells, 80% of the cells in the ganglion cell layer, and none of the pyriform Ab amacrine cells or cells in the inner plexiform layer. We suggest that the polyform cells compose the major population of GABAergic amacrine cells in the goldfish retina, rather than the pyriform Ab cells. GABAergic displaced amacrine cells or ganglion cells are also indicated by our data. The implications of these data with regard to the physiology of the goldfish retina are discussed as well.