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Embryonic development of monoaminergic neurons in the chick retina
Author(s) -
Kato S.,
Negishi K.,
Teranishi T.
Publication year - 1984
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.902240311
Subject(s) - monoaminergic , retina , biology , inner nuclear layer , embryonic stem cell , retinal , dopamine , monoamine neurotransmitter , microbiology and biotechnology , dopaminergic , anatomy , endocrinology , medicine , neuroscience , serotonin , receptor , biochemistry , gene
By means of a histofluorescence technique, embryonic and postnatal development of monoaminergic neurons was followed in the chicken retina with or without intravitreal injection of monoamines 30–60 minutes before eye removal. Fluorescent cells were tentatively classified into five subsets with respect to the soma shape, localization, migration of somata during retinal development, uptake capacity (color and intensity in fluorescence), and sensitivity to neurotoxins. The five subsets of cells were endogenous dopaminergic (DA), catecholamine‐accumulating (CA), indoleamine‐accumulating (IA), CA‐bipolarlike, and IA‐bipolarlike cells. Greenish endogenous DA‐cells first appeared at the 14–15th embryonic day. The cell body of DA‐cells was initially fusiform and located slightly distal to the innermost level of the inner nuclear layer (INL). They became round or oval and migrated to the innermost level of the INL by day 20. Both large and small bottle‐shaped CA‐cells were visualized at an intermediate portion of INL by intravitreal injection of exogenously applied dopamine or noradrenaline (1–2 m̈g/eye) at day 10. Large bottle‐shaped cells, like the DA cells, changed to round or oval and migrated to the innermost level of the INL by day 20. On the other hand, small bottle‐shaped CA‐cells retained their cell shape and location in the INL as retinal development progressed. Therefore, the large bottle‐shaped CA‐cells seen in an early developmental stage correspond to the DA‐cells. IA‐cells were visualized one or two cell rows outward in the INL first at day 13–14 by intravitreal injection of 5,6‐dihydroxytryptamine or 5‐hydroxytryptamine (1–5 m̈g). These cells did not change their relative location during development. The emergence of the catecholaminergic uptake property (DA‐ and CA‐cells) may slightly precede that of the indoleaminergic property (IA‐cells) in the embryonic stage. When excess doses (10–20 m̈g) of monoamines were intravitreally injected, a large number of CA‐ and IA‐bipolarlike cells became visible, peaking in number probably during a certain embryonic period (after day 14–15). DA‐ and CA‐cells could be destroyed by intravitreal injection of 6‐hydroxydopamine (15 m̈g, twice) and IA‐cells could be selectively eliminated by intravitreal 5,7‐dihydroxytryptamine, whereas CA‐ and IA‐bipolarlike cells were not influenced by the dose of neurotoxins used.