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Fine structural localization of a new Purkinje cell‐specific glycoprotein subunit: Immunoelectron microscopical study
Author(s) -
Langley O. K.,
Reeber A.,
Vincendon G.,
Zanetta J. P.
Publication year - 1982
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.902080404
Subject(s) - purkinje cell , immunoelectron microscopy , biology , endoplasmic reticulum , cerebellum , cytoplasm , granule cell , microbiology and biotechnology , electron microscope , immunoperoxidase , protein subunit , axon , biophysics , biochemistry , immunohistochemistry , neuroscience , central nervous system , antibody , immunology , dentate gyrus , physics , monoclonal antibody , gene , optics
The localization of a new low molecular weight glycoprotein subunit in cerebellum was studied with the aid of the indirect immunohistochemical method at the electron microscope level. The specificity of this molecule for Purkinje cells in the rat cerebellum suggested by earlier light microscope studies was confirmed. Immunoperoxidase reaction product indicative of the presence of this glycoprotein was exclusively limited to Purkinje cells, attached to the plasma membrane, membranes of the rough endoplasmic reticulum, the cytoplasmic surface of the nuclear membrane, and on polyribosomes. The antigen was found throughout the whole cell, except for the nucleus, and enabled Purkinje cell processes to be traced in the molecular layer, in the granule cell layer (including the nerve terminals in this layer), and to their axon terminals in the deep nuclei. The fine structural localization is discussed with reference to the known properties of this antigen.