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Androgen‐Concentrating cells in the periventricular brain of the female rhesus monkey
Author(s) -
Sheridan Peter J.,
Hagino Nobuyoshi,
Weaker Frank J.
Publication year - 1982
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.902070109
Subject(s) - forebrain , biology , dihydrotestosterone , primate , androgen , endocrinology , amygdala , medicine , hypothalamus , hippocampus , limbic system , basal forebrain , central nervous system , testosterone (patch) , endocrine system , preoptic area , neuroscience , hormone
Although androgens act on the primate central nervous system to modulate both endocrine functions and a number of limbic‐related behaviors, little is known about the anatomical location of the neurons which sequester these steroids in primates. To determine the prime location of these androgen‐concentrating neurons in the forebrain of the primate, we injected three castrated female rhesus monkeys in the femoral vein with 1 μg of 5α‐dihydro (1, 2, 4, 5, 6, 7‐ 3 H) testosterone ( 3 H‐DHT, 107 Ci/mmole) per kg of body weight. One of these animals also received an IV injection of 100 μg/kg body weight of unlabeled dihydrotestosterone (DHT) to serve as a control. One hour after the injection of 3 H‐DHT we rapidly exsanguinated each animal. The forebrain was sliced and blocks containing the amygdala, diencephalon, frontal pole, and hippocampus were frozen and stored in liquid nitrogen until processing. The tissue was then processed for autoradiography. A specific topographic pattern of nuclear concentration of DHT or one of its metabolites was obtained in neurons of the basal hypothalamus, preoptic region, amygdala, and hippocampus. This pattern was similar to that found in rodent species.