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The emergence, localization, and maturation of neurotransmitter systems during development of the retina in Xenopus laevis . III. Dopamine
Author(s) -
Sarthy P. Vijay,
Rayborn Mary E.,
Hollyfield Joe G.,
Lam Dominic M. K.
Publication year - 1981
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.901950405
Subject(s) - xenopus , biology , dopamine , neurotransmitter , retina , neuroscience , anatomy , microbiology and biotechnology , central nervous system , biochemistry , gene
The uptake, synthesis, and release of dopamine was studied in retinas of Xenopus laevi . In the tadpole and adult retina, 3 H‐dopamine is accumulated by cells located in the inner nuclear layer. Retinas preloaded with 3 H‐dopamine release this compound in response to high K + concentrations in the medium. This release is probably Ca ++ ‐dependent as it is inhibited by Co ++ in the medium. Adult retinas are also capable of synthesizing 3 H‐dopamine from 3 H‐tyrosine. The appearance and maturation of these dopaminergic properties were followed during retinal development. Our data indicate that synthesis of dopamine can first be detected as early as stage 35/36 whereas uptake of dopamine first occurs at stage 43. K + ‐stimulated release of preloaded 3 H‐dopamine from putative dopaminergic neurons is, however, not evident until stage 46. These results show that similar to the development of GABA‐ergic and glycinergic properties, the uptake, synthesis, and release mechanisms for dopamine emerge at different stages during retinal differentiation in Xenopus Laevis .