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Opening of tight junctions in cerebral endothelium. II. Effect of pressure‐pulse induced acute arterial hypertension
Author(s) -
Nagy Zoltan,
Mathieson Gordon,
Hüttner Istvan
Publication year - 1979
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.901850308
Subject(s) - extravasation , evans blue , horseradish peroxidase , endothelium , blood–brain barrier , blood pressure , biology , vascular permeability , tight junction , pulse pressure , anatomy , anesthesia , medicine , endocrinology , pathology , central nervous system , biochemistry , enzyme
Acute arterial hypertension was produced in male Wister rats by pressure pulse through the right internal carotid artery. The pressure pulse was induced by infusion of physiological saline as a bolus, at a rate of 0.63 ml per second by syringe pump. Evans blue (Eb) was used to visualize the areas of blood‐brain barrier opening. Intravenously injected horseradish peroxidase (HRP) was used to study the ultrastructural basis of permeability changes in cerebral endothelium. Eb outlined circumscribed areas of blood‐brain barrier opening. HRP extravasation was found mainly around small arteries. The capillary network was effected to a much lesser extent. Electron microscopy showed that HRP crossed the endothelial cell layer by intercellular routes. Glutaraldehyde fixed brain samples permeated with colloidal lanthanum supported these observations.