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Opening of tight junctions in cerebral endothelium. I. Effect of hyperosmolar mannitol infused through the internal carotid artery
Author(s) -
Nagy Zoltan,
Pappius Hanna M.,
Mathieson Gordon,
Hüttner Istvan
Publication year - 1979
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.901850307
Subject(s) - horseradish peroxidase , mannitol , endothelium , biology , tonicity , blood–brain barrier , tight junction , internal carotid artery , cerebral circulation , anatomy , cerebral arteries , medicine , central nervous system , neuroscience , endocrinology , biochemistry , enzyme
Abstract Infusion of 1.8 M mannitol solution into the internal corotid artery of Wistar rats allows horseradish peroxidase (HRP) to cross cerebral vascular endothelium via intercellular pathways. This was made evident by density gradients of HRP observed in consecutive tight junctional compartments following in vivo administration, and by the passage of colloidal lanthanum through junctional membrane appositions of glutaradehyde fixed cerebral endothelium. Three hours after mannitol infusion, small no‐capillary vascular segments were still focally permeable to HRP. Twenty‐four hours after the osmotic insult, the tracer was absent in the affected hemisphere. Water and electrolyte essays indicated absence of brain edema during the period of observation of blood‐brain barrier opening.