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Human brain in tissue culture. I. Acquisition, initial processing, and establishment of brain cell cultures
Author(s) -
Gilden Donald H.,
Devlin Mary,
Wroblewska Zofia,
Friedman Harvey,
Rorke Lucy Balian,
Santoli Daniela,
Koprowski Hilary
Publication year - 1975
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.901610302
Subject(s) - biology , in vitro , cell culture , tissue culture , neuroscience , autopsy , pathology , brain cell , human brain , brain tissue , biopsy , brain biopsy , central nervous system , medicine , genetics
This paper details the in vitro techniques used to establish cells in culture from the brains of 40 patients, most of whom had chronic neurologic disease. The clinical and pathologic features of these patients are given. The success in establishing cell lines was dependent upon the origin of tissue (biopsy vs. autopsy), the site of removal from the brain, and various environmental and technical manipulations in vitro.