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Neuroglial response to sciatic neurectomy. I. Light microscopy and autoradiography ,
Author(s) -
Kerns James M.,
Hinsman Edward J.
Publication year - 1973
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.901510303
Subject(s) - microglia , neuroglia , biology , sciatic nerve , anatomy , pathology , neuroscience , central nervous system , immunology , medicine , inflammation
The response of oligodendrocytes, astrocytes, microglia, and pericytes to sciatic neurectomy in young adult rats was studied from 1μ Epon sections for quantitative cell counts and autoradiography. Two hours after an IP injection of 3 H‐thymidine animals were killed by aldehyde perfusion at survival periods of 2, 4, 7, and 14 days postoperative (dpo). At 2 dpo there was a marked increase in the number of labeled microglial cell nuclei and mitotic figures on the experimental side. This activation appeared to involve most of the “resting” microglia, which comprise about 6% of the total neuroglia in the normal ventral horn. A significant numerical increase (over fourfold) in perineuronal and total microglia was seen at 4 dpo followed by a gradual decline. Other findings include minimal chromatolysis in ventral horn neurons, contralateral effects, and a slight proliferative response in and around the central canal. A response was not apparent in the other neuroglia, pericytes, or mononuclear leukocytes. The relationship of the microglia to the multipotential glia of neuroectodermal origin is considered.