Premium
Genesis of glioblasts in the human spinal cord as revealed by Feulgen cytophotometry
Author(s) -
Fujita S.
Publication year - 1973
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.901510103
Subject(s) - spinal cord , biology , gliogenesis , anatomy , embryo , subarachnoid space , pathology , embryogenesis , neuroscience , microbiology and biotechnology , cerebrospinal fluid , stem cell , medicine , neural stem cell
To study gliogenesis in the spinal cord, Feulgen cytophotometry was carried out on cells in the cervical cord of human embryos ranging from 7–45 mm in crown‐rump length. Glioblasts synthesizing DNA are first detectable at eight‐week‐stage in the mantle and marginal zones in the basal plate and immediately thereafter in the alar plate. Coincidentally the matrix layer changes into a single‐layered ependymal lining. This pattern of the incipient stage of gliogenesis in the human spinal cord closely resembles that of the chick embryo. Silver carbonate staining of the spinal cord at this stage showed diverse morphology of the glioblasts at their earliest stage of differentiation. The pattern of glioblast formation coincides with that of the genesis of the so‐called microglioblasts so far reported, not only in the space‐time relationships in embryogenesis but also in the morphology of the cells occurring at this stage. It is concluded that the so‐called genesis of the microglia in the spinal cord is nothing but the stage of glioblast formation from the matrix cells.