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Proliferation and turnover of glial cells in the forebrain of young adult mice as studied by repeated injections of 3 H‐thymidine over a prolonged period of time
Author(s) -
KrausRuppert R.,
Laissue J.,
Bürki H.,
Odartchenko N.
Publication year - 1973
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.901480206
Subject(s) - subependymal zone , thymidine , period (music) , biology , forebrain , cerebral cortex , neuroglia , cortex (anatomy) , dna synthesis , precursor cell , cell , endocrinology , neuroscience , medicine , central nervous system , dna , biochemistry , physics , acoustics
The magnitude of glial cell renewal was studied on young adult mice using repeated intraperitoneal injections of 3 H‐thymidine every eight hours over a period of 30 days. Mean labeling indices one hour after the last injection were as follows: Glial cells of the subependymal layer of the lateral ventricle, 61.5%; oligodendrocytes (various sites), 24 to 36.2%; astrocytes (various sites), 14.3 to 30.8%, and satellites in the cerebral cortex, 32.7%. Since DNA synthesis time of the proliferating, immature glial cells is unknown and may be shorter than the time interval of eight hours chosen for repeated injections of 3 H‐thymidine, these results are interpreted as representing minimum values for turnover, during 30 days, of the various cell types in different areas of the forebrain. The significance of a marked proliferative activity of the glial cells as related to differentiation and possible migration of subependymal cells, is discussed.