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The in vitro effect of the nerve growth factor on chick embryo spinal ganglia: An electron microscopic evaluation
Author(s) -
Weis Peddrick
Publication year - 1971
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.901410110
Subject(s) - neuroblast , biology , nerve growth factor , neurite , embryo , myelin , microbiology and biotechnology , anatomy , electron microscope , in vivo , neuroscience , in vitro , central nervous system , neurogenesis , biochemistry , physics , receptor , optics
Chick embryo spinal ganglia, cultured with and without he nerve growth factor (NGF), were compared by means of electron microscopy with ganglia maintained in vivo for an equal period of time. The results confirm an earlier report that NGF maintains and stimulates only the later‐differentiating mesiodorsal neuroblasts. However, although these cells are stimulated to grow neurites four days prematurely, cytoplasmic maturation does not accompany the outgrowth. The more‐differentiated ventrolateral neuroblasts all show some signs of degeneration after culturing with or without NGF. Those cells which appear normal in the light microscope failed to continue development like their in vivo counterparts and are seen to have myelin figures, marked indentation of the nuclear membrane, and chromatin clumping. Crystalloid structures originally observed by Crain et al. ('64) are demonstrated to be ribosomal crystals and are found only in hyperchromatic degenerated neuroblasts. The satellite cells do not continue to develop in vitro . The process of encapsulatin neuroblasts ceases in both the mesiodorsal and ventrolateral areas of the ganglia. The interrelationship of satellite cell and neuroblast develoment is discussed.