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Cytoplasmic basophilia in the nerve cells of the cerebral cortex. VI. The human temporal cortex
Author(s) -
Pakkenberg Henning
Publication year - 1967
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.901290408
Subject(s) - basophilia , biology , temporal lobe , cerebral cortex , cortex (anatomy) , nerve cells , nissl body , cytoplasm , staining , human brain , temporal cortex , anatomy , extinction (optical mineralogy) , pathology , neuroscience , microbiology and biotechnology , epilepsy , medicine , paleontology , genetics , immunology
An attempt is made to evaluate the metabolic functional pattern in the nerve cells of a human temporal lobe by determining the cytoplasmic basophilia. Following gallocyanin staining (RNA staining), the extinction is measured at two points in each cell by means of a cytophotometer at 570 nm. The section thickness is determined, and measurements are made only on those cells which are known with certainty to be cut in both section surfaces. A total of 2000 cells is measured, distributed throughout five sites over the temporal lobe. In each layer, mean extinction values are found which do not deviate significantly from each other in the regions measured. The results are compared with previous findings from the frontal lobe of the same brain, and the conclusion is reached that the distribution of chromophobic, chromophilic and chromoneutral cells must be taken as expressing the fact that a majority of cells are in moderate activity, and that two small groups are in a somewhat greater metabolic activity. The distribution pattern is the same in different regions of the isocortex, possibly a result of the intimate communications characteristic of the cerebral cortex.