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Filamentous and tubular structures in mammalian axoplasm. An electron microscopic study ,
Author(s) -
Cravioto Humberto
Publication year - 1966
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.901260307
Subject(s) - uranyl acetate , axoplasm , glutaraldehyde , phosphotungstic acid , biology , ultrastructure , araldite , biochemistry , electron microscope , acetone , biophysics , anatomy , chromatography , chemistry , axon , organic chemistry , physics , adhesive , layer (electronics) , optics , catalysis
A neurofibrillar and neurotubular ultrastructure of mammalian axoplasm has been observed consistently after glutaraldehyde fixation. The embedding media (Epon, Maraglas, Vestopal W, Araldite), dehydration procedure (alcohol, acetone) and counter‐strain used (lead citrate, uranyl acetate, phosphotungstic acid) played no significant role in the selective demonstration of neurotubules or neurofibrils. It was suggested that the neurotubules may form part of the neurofibrillar apparatus described by Cajal.