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DARPP‐32 distinguishes a subset of adult‐born neurons in zebra finch HVC
Author(s) -
Aronowitz Jake V.,
Kirn John R.,
Pytte Carolyn L.,
Aaron Gloster B.
Publication year - 2022
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.25245
Subject(s) - zebra finch , taeniopygia , biology , catecholaminergic , tyrosine hydroxylase , neuroscience , neun , dopamine , immunohistochemistry , immunology
Adult male zebra finches ( Taeniopygia guttata ) continually incorporate adult‐born neurons into HVC, a telencephalic brain region necessary for the production of learned song. These neurons express activity‐dependent immediate early genes (e.g., zenk and c‐fos ) following song production, suggesting that these neurons are active during song production. Half of these adult‐born HVC neurons (HVC NNs) can be backfilled from the robust nucleus of the arcopallium (RA) and are a part of the vocal motor pathway underlying learned song production, but the other half do not backfill from RA, and they remain to be characterized. Here, we used cell birth‐dating, retrograde tract tracing, and immunofluorescence to demonstrate that half of all HVC NNs express the phosphoprotein DARPP‐32, a protein associated with dopamine receptor expression. We also demonstrate that DARPP‐32+ HVC NNs are contacted by tyrosine hydroxylase immunoreactive fibers, suggesting that they receive catecholaminergic input, have transiently larger nuclei than DARPP‐32‐neg HVC NNs, and do not backfill from RA. Taken together, these findings help characterize a group of HVC NNs that have no apparent projections to RA and so far have eluded positive identification other than HVC NN status.