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Novel circular RNA 2960 contributes to secondary damage of spinal cord injury by sponging miRNA ‐124
Author(s) -
Chen Jun,
Fu Bin,
Bao Jing,
Su Rong,
Zhao Haoning,
Liu Zhongtao
Publication year - 2021
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.25030
Subject(s) - microrna , kegg , biology , downregulation and upregulation , circular rna , spinal cord injury , gene , gene ontology , microbiology and biotechnology , gene expression , apoptosis , cancer research , spinal cord , genetics , neuroscience
Abstract Recent studies have shown that circular RNAs (circRNAs) are involved in many human diseases, but their roles in secondary damage after spinal cord injury (SCI) remain unclear. In the current study circRNA sequencing was performed in the damaged tissues of SCI rats on the seventh day after injury, and related molecular mechanisms were investigated. Quantitative PCR validations of molecules that exhibited significantly altered expression in SCI mice were performed. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses were conducted to assess differentially expressed circRNAs. A novel circRNA‐2960 was the most significantly upregulated in the SCI group. It could downregulate its target molecule miRNA‐124, then exacerbate the inflammatory response and induce apoptosis at the lesion site. Disrupting circRNA‐2960 expression promoted recovery of tissues affected by secondary SCI damage. The results of the present study may provide new insight into the mechanisms of secondary injury in SCI, and a new molecular marker for the diagnosis and treatment of SCI.