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LacZ‐reporter mapping of Dlx5 / 6 expression and genoarchitectural analysis of the postnatal mouse prethalamus
Author(s) -
Puelles Luis,
Diaz Carmen,
Stühmer Thorsten,
Ferran José L.,
Martínezde la Torre Margaret,
Rubenstein John L. R.
Publication year - 2021
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.24952
Subject(s) - biology , dlx5 , lac operon , population , enhancer , microbiology and biotechnology , genetics , transcription factor , gene expression , gene , homeobox , demography , sociology
We present here a thorough and complete analysis of mouse P0‐P140 prethalamic histogenetic subdivisions and corresponding nuclear derivatives, in the context of local tract landmarks. The study used as fundamental material brains from a transgenic mouse line that expresses LacZ under the control of an intragenic enhancer of Dlx5 and Dlx6 ( Dlx5 / 6‐LacZ ). Subtle shadings of LacZ signal, jointly with pan‐DLX immunoreaction, and several other ancillary protein or RNA markers, including Calb2 and Nkx2.2 ISH (for the prethalamic eminence, and derivatives of the rostral zona limitans shell domain, respectively) were mapped across the prethalamus. The resulting model of the prethalamic region postulates tetrapartite rostrocaudal and dorsoventral subdivisions, as well as a tripartite radial stratification, each cell population showing a characteristic molecular profile. Some novel nuclei are proposed, and some instances of potential tangential cell migration were noted.