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Gad1‐promotor‐driven GFP expression in non‐GABAergic neurons of the nucleus endopiriformis in a transgenic mouse line
Author(s) -
Riedemann Sophie,
Sutor Bernd,
Bergami Matteo,
Riedemann Therese
Publication year - 2019
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.24673
Subject(s) - biology , transgene , gabaergic , green fluorescent protein , genetically modified mouse , population , microbiology and biotechnology , neuroscience , nucleus , reporter gene , neuron , gene expression , gene , genetics , inhibitory postsynaptic potential , demography , sociology
Transgenic animals have become a widely used model to identify and study specific cell types in whole organs. Promotor‐driven reporter gene labeling of the cells under investigation has promoted experimental efficacy to a large degree. However, rigorous assessment of transgene expression specificity in these animal models is highly recommended to validate cellular identity and to isolate potentially mislabeled cell populations. Here, we report on one such mislabeled neuron population in a widely used transgenic mouse line in which GABAergic somatostatin‐expressing interneurons (SOM pos INs) are labeled by eGFP (so‐called GIN mouse, FVB‐Tg(GadGFP)45704Swn/J). These neurons represent a subpopulation of all SOM pos INs. However, we report here on GFP labeling of non‐GABAergic neurons in the nucleus endopiriformis of this mouse line.