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Transcriptome response to infraorbital nerve transection in the gonadally intact male rat barrel cortex: RNA‐seq
Author(s) -
Orczyk John J.,
Sethia Rishabh,
Doster Dominique,
Garraghty Preston E.
Publication year - 2015
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.23831
Subject(s) - biology , infraorbital nerve , barrel cortex , transcriptome , neurotrophin , microdissection , gene expression , brain derived neurotrophic factor , laser capture microdissection , messenger rna , neurotrophic factors , rna , microbiology and biotechnology , cortex (anatomy) , endocrinology , medicine , neuroscience , anatomy , gene , sensory system , receptor , biochemistry
The effects of infraorbital nerve (ION) transection on gene expression in the adult male rat barrel cortex were investigated using RNA sequencing. After a 24‐hour survival duration, 98 genes were differentially regulated by ION transection. Differentially expressed genes suggest changes in neuronal activity, excitability, and morphology. The production of mRNA for neurotrophins, including brain‐derived neurotrophin factor (BNDF), was decreased following ION transection. Several potassium channels showed decreased mRNA production, whereas a sodium channel (Na V β4) associated with burst firing showed increased mRNA production. The results may have important implications for phantom‐limb pain and complex regional pain syndrome. Future experiments should determine the extent to which changes in RNA result in changes in protein expression, in addition to utilizing laser capture microdissection techniques to differentiate between neuronal and glial cells. J. Comp. Neurol. 524:152–159, 2016. © 2015 Wiley Periodicals, Inc.