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Aging Drosophila melanogaster display altered pre‐ and postsynaptic ultrastructure at adult neuromuscular junctions
Author(s) -
Wagner Nicole,
Laugks Ulrike,
Heckmann Manfred,
Asan Esther,
Neuser Kirsa
Publication year - 2015
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.23798
Subject(s) - postsynaptic potential , biology , postsynaptic density , synaptic vesicle , neuromuscular junction , active zone , ultrastructure , neuroscience , synaptic plasticity , microbiology and biotechnology , glutamate receptor , excitatory postsynaptic potential , vesicle , anatomy , inhibitory postsynaptic potential , receptor , biochemistry , membrane
Although age‐related changes in synaptic plasticity are an important focus within neuroscience, little is known about ultrastructural changes of synaptic morphology during aging. Here we report how aging affects synaptic ultrastructure by using fluorescence and electron microscopy at the adult Drosophila neuromuscular junction (NMJ) of ventral abdominal muscles. Mainly four striking morphological changes of aging NMJs were revealed. 1) Bouton size increases with proportionally rising number of active zones (AZs). 2) Synaptic vesicle density at AZs is increased in old flies. 3) Late endosomes, cisternae, and multivesicular bodies accumulate in the presynaptic terminal, and vesicles accumulate between membranes of the terminal bouton and the subsynaptic reticulum. 4) The electron‐dense pre‐ and postsynaptic apposition is expanded in aging NMJs, which is accompanied by an expansion of the postsynaptic glutamate receptor fields. These findings suggest that aging is possibly accompanied by impaired synaptic vesicle release and recycling and a potentially compensatory expansion of AZs and postsynaptic densities. J. Comp. Neurol. 523:2457–2475, 2015. © 2015 Wiley Periodicals, Inc.