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Distribution and innervation of putative arterial chemoreceptors in the bullfrog ( Rana catesbeiana )
Author(s) -
Reyes Catalina,
Fong Angelina Y.,
Brink Dee L.,
Milsom William K.
Publication year - 2014
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.23640
Subject(s) - glomus cell , biology , bullfrog , carotid body , acetylcholine , vesicular acetylcholine transporter , anatomy , cholinergic , rana , medicine , monoaminergic , neurotransmitter , serotonin , endocrinology , 5 ht receptor , choline acetyltransferase , receptor , central nervous system , stimulation , biochemistry
Peripheral arterial chemoreceptors have been located previously in the carotid labyrinth, the aortic arch, and the pulmocutaneous artery of frogs. In the present study we used cholera toxin B neuronal tract tracing and immunohistochemical markers for cholinergic cells (vesicular acetylcholine transporter [VAChT]), tyrosine hydroxylase (TH), and serotonin (5HT) to identify putative O 2 ‐sensing cells in Rana catesbeiana . We found potential O 2 ‐sensing cells in all three vascular areas innervated by branches of the vagus nerve, whereas only cells in the carotid labyrinth were innervated by the glossopharyngeal nerve. Cells containing either 5HT or TH were found in all three sites, whereas cells containing both neurotransmitters were found only in the carotid labyrinth. Cell bodies containing VAChT were not found at any site. The morphology and innervation of putative O 2 ‐sensing cells were similar to those of glomus cells found in other vertebrates. The presence of 5HT‐ and TH‐immunoreactive cells in the aorta, pulmocutaneous artery, and carotid labyrinth appears to reflect a phylogenetic transition between the major neurotransmitter seen in the putative O2‐sensing cells of fish (5HT) and those found in the glomus cells of mammals (acetylcholine, adenosine, and catecholamines). J. Comp. Neurol. 522:3754–3774, 2014. © 2014 Wiley Periodicals, Inc.