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Quantitative analysis of axon bouton distribution of subthalamic nucleus neurons in the rat by single neuron visualization with a viral vector
Author(s) -
Koshimizu Yoshinori,
Fujiyama Fumino,
Nakamura Kouichi C.,
Furuta Takahiro,
Kaneko Takeshi
Publication year - 2013
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.23277
Subject(s) - subthalamic nucleus , neuroscience , globus pallidus , axon , basal ganglia , biology , striatum , putamen , medium spiny neuron , neuron , substantia nigra , direct pathway of movement , anatomy , deep brain stimulation , parkinson's disease , central nervous system , dopamine , pathology , medicine , disease , dopaminergic
The subthalamic nucleus (STN) of the basal ganglia plays a key role in motor control, and STN efferents are known to mainly target the external segment of the globus pallidus (GPe), entopeduncular nucleus (Ep), and substantia nigra (SN) with some axon collaterals to the other regions. However, it remains to be clarified how each STN neuron projects axon fibers and collaterals to those target nuclei of the STN. Here we visualized the whole axonal arborization of single STN neurons in the rat brain by using a viral vector expressing membrane‐targeted green fluorescent protein, and examined the distribution of axon boutons in those target nuclei. The vast majority (8–9) of 10 reconstructed STN neurons projected to the GPe, SN, caudate‐putamen (CPu), and Ep, which received, on average ± SD, 457 ± 425, 400 ± 347, 126 ± 143, and 106 ± 100 axon boutons per STN neuron, respectively. Furthermore, the density of axon boutons in the GPe was highest among these nuclei. Although these target nuclei were divided into calbindin‐rich and ‐poor portions, STN projection showed no exclusive preference for those portions. Since STN neurons mainly projected not only to the GPe, SN, and Ep but also to the CPu, the subthalamostriatal projection might serve as a positive feedback path for the striato‐GPe‐subthalamic disinhibitory pathway, or work as another route of cortical inputs to the striatum through the corticosubthalamostriatal disynaptic excitatory pathway. J. Comp. Neurol. 521:2125–2146, 2013. © 2012 Wiley Periodicals, Inc.