Premium
Doublecortin and doublecortin‐like are expressed in overlapping and non‐overlapping neuronal cell population: Implications for neurogenesis
Author(s) -
Saaltink DirkJan,
Håvik Bjarte,
Verissimo Carla S.,
Lucassen P.J.,
Vreugdenhil Erno
Publication year - 2012
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.23172
Subject(s) - doublecortin , neurogenesis , biology , neuroscience , rostral migratory stream , subventricular zone , olfactory bulb , neun , neural stem cell , population , microbiology and biotechnology , dentate gyrus , hippocampus , stem cell , central nervous system , immunology , immunohistochemistry , demography , sociology
We have characterized the expression of doublecortin‐like (DCL), a microtubule‐associated protein involved in embryonic neurogenesis that is highly homologous to doublecortin (DCX), in the adult mouse brain. To this end, we developed a DCL‐specific antibody and used this to compare DCL expression with DCX. In the neurogenic regions of the adult brain like the subventricular zone (SVZ), the rostral migratory stream (RMS), the olfactory bulb (OB), and the hippocampus, DCL colocalizes with DCX in immature neuronal cell populations. In contrast to DCX, we also found high DCL expression in three other brain regions with suspected neurogenesis or neuronal plasticity. First, the radial glia‐like, hypothalamic tanycytes show high DCL expression that partly colocalizes with the neural stem cell marker vimentin. Second, DCL expression is found in cells of the suprachiasmatic nucleus (SCN), which lacks expression of the adult neuron marker NeuN. Third, a novel region exhibiting DCL expression is part of the olfactory tubercle where DCL is found in the neuropil of the islands of Calleja (ICj). Our findings define DCL as a novel marker for specific aspects of adult neurogenesis, which partly overlap with DCX. In addition, we propose unique roles for DCL in adult neurogenesis and we suggest high levels of neuronal plasticity in tanycytes, SCN, and ICj. J. Comp. Neurol. 520:2805–2823, 2012. © 2012 Wiley Periodicals, Inc.