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Laser‐capture microdissection and transcriptional profiling of the dorsomedial nucleus of the hypothalamus
Author(s) -
Lee Syann,
Bookout Angie L.,
Lee Charlotte E.,
Gautron Laurent,
Harper Matthew J.,
Elias Carol F.,
Lowell Bradford B.,
Elmquist Joel K.
Publication year - 2012
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.23116
Subject(s) - laser capture microdissection , biology , microdissection , gene expression , gene expression profiling , gene , hypothalamus , in situ hybridization , nucleus , genetics , computational biology , neuroscience
Identifying neuronal molecular markers with restricted patterns of expression is a crucial step in dissecting the numerous pathways and functions of the brain. While the dorsomedial nucleus of the hypothalamus (DMH) has been implicated in a host of physiological processes, current functional studies have been limited by the lack of molecular markers specific for DMH. Identification of such markers would facilitate the development of mouse models with DMH‐specific genetic manipulations. Here we used a combination of laser‐capture microdissection (LCM) and gene expression profiling to identify genes that are highly expressed within the DMH relative to adjacent hypothalamic regions. Six of the most highly expressed of these genes, Gpr50, 4930511J11Rik, Pcsk5, Grp, Sulf1 , and Rorβ , were further characterized by real‐time polymerase chain reaction (PCR) analysis and in situ hybridization histochemistry. The genes identified in this article will provide the basis for future gene‐targeted approaches for studying DMH function. J. Comp. Neurol. 520:3617–3632, 2012. © 2012 Wiley Periodicals, Inc.