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Electron tomographic analysis of synaptic ultrastructure
Author(s) -
Burette Alain C.,
Lesperance Thomas,
Crum John,
Martone Maryann,
Volkmann Niels,
Ellisman Mark H.,
Weinberg Richard J.
Publication year - 2012
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.23067
Subject(s) - biology , neocortex , neuroscience , synapse , excitatory postsynaptic potential , ultrastructure , electron tomography , osmium tetroxide , electron microscope , electrophysiology , anatomy , scanning transmission electron microscopy , optics , inhibitory postsynaptic potential , physics
Synaptic function depends on interactions among sets of proteins that assemble into complex supramolecular machines. Molecular biology, electrophysiology, and live‐cell imaging studies have provided tantalizing glimpses into the inner workings of the synapse, but fundamental questions remain regarding the functional organization of these “nano‐machines.” Electron tomography reveals the internal structure of synapses in three dimensions with exceptional spatial resolution. Here we report results from an electron tomographic study of axospinous synapses in neocortex and hippocampus of the adult rat, based on aldehyde‐fixed material stabilized with tannic acid in lieu of postfixation with osmium tetroxide. Our results provide a new window into the structural basis of excitatory synaptic processing in the mammalian brain. J. Comp. Neurol. 520:2697–2711, 2012. © 2012 Wiley Periodicals, Inc.