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Migration of neuronal precursors from the telencephalic ventricular zone into the olfactory bulb in adult zebrafish
Author(s) -
Kishimoto Norihito,
AlfaroCervello Clara,
Shimizu Kohei,
Asakawa Kazuhide,
Urasaki Akihiro,
aka Shigenori,
Kawakami Koichi,
GarciaVerdugo Jose Manuel,
Sawamoto Kazunobu
Publication year - 2011
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.22722
Subject(s) - olfactory bulb , zebrafish , cerebrum , biology , rostral migratory stream , subventricular zone , neurogenesis , neuroscience , anatomy , fate mapping , embryonic stem cell , microbiology and biotechnology , central nervous system , neural stem cell , stem cell , progenitor cell , biochemistry , gene
In the brain of adult mammals, neuronal precursors are generated in the subventricular zone in the lateral wall of the lateral ventricles and migrate into the olfactory bulbs (OBs) through a well‐studied route called the rostral migratory stream (RMS). Recent studies have revealed that a comparable neural stem cell niche is widely conserved at the ventricular wall of adult vertebrates. However, little is known about the migration route of neuronal precursors in nonmammalian adult brains. Here, we show that, in the adult zebrafish, a cluster of neuronal precursors generated in the telencephalic ventricular zone migrates into the OB via a route equivalent to the mammalian RMS. Unlike the mammalian RMS, these neuronal precursors are not surrounded by glial tubes, although radial glial cells with a single cilium lined the telencephalic ventricular wall, much as in embryonic and neonatal mammals. To observe the migrating neuronal precursors in living brain tissue, we established a brain hemisphere culture using a zebrafish line carrying a GFP transgene driven by the neurogenin1 ( ngn1 ) promoter. In these fish, GFP was observed in the neuronal precursors migrating in the RMS, some of which were aligned with blood vessels. Numerous ngn1:gfp ‐positive cells were observed migrating tangentially in the RMS‐like route medial to the OB. Taken together, our results suggest that the RMS in the adult zebrafish telencephalon is a functional migratory pathway. This is the first evidence for the tangential migration of neuronal precursors in a nonmammalian adult telencephalon. J. Comp. Neurol. 519:3549–3565, 2011. © 2011 Wiley‐Liss, Inc.