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Expression profile of PTPIP51 in mouse brain
Author(s) -
Koch P.,
Viard M.,
Stenzinger A.,
Brobeil A.,
Tag C.,
Steger K.,
Wimmer M.
Publication year - 2009
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.22201
Subject(s) - biology , neuroscience , expression (computer science) , computer science , programming language
This study demonstrates the expression of the novel protein protein tyrosine phophatase‐interacting protein 51 (PTPIP51) in mammalian brain tissue. Serial sections of the whole adult mouse brain were analyzed for PTPIP51 protein and mRNA by immunohistochemistry, immunoblotting, RT‐PCR, and in situ hybridization. Recent investigations by Yu et al. (2008) describe PTPIP51 as being capable of activating Raf‐1, thereby modulating the MAPK pathway. The role of Raf‐1, as well as of 14‐3‐3, in neurological disorders is well established. PTPIP51 expression was confined to neurons in the following structures: the piriform cortex and their connections to the anterior commissure, nucleus accumbens, paraventricular and supraoptical nuclei, neurohypophysis, superior colliculus, genu of facialis nerve, spinal trigeminal tract, inferior cerebellar peduncle, and cerebellum. In the cerebellum, a subpopulation of Purkinje cells and their dendrites was strongly PTPIP51 positive. Moreover, PTPIP51 was found to be colocalized with vasopressin and its transport protein neurophysin II in the neuroendocrine nuclei and their connections to the neurohypophysis. The data presented here suggest a role of PTPIP51 in neuronal homeostasis, axonal growth, and transport. J. Comp. Neurol. 517:892–905, 2009. © 2009 Wiley‐Liss, Inc.