Synaptic organization of the tectorecipient zone of the rat lateral posterior nucleus

Abstract Dorsal thalamic nuclei have been categorized as either “first‐order” nuclei that gate the transfer of relatively unaltered signals from the periphery to the cortex or “higher order” nuclei that transfer signals from one cortical area to another. To classify the tectorecipient lateral posterior (LPN), we examined the synaptic organization of tracer‐labeled cortical and tectal terminals and terminals labeled with antibodies against the type 1 and type 2 vesicular glutamate transporters (vGLUT1 and vGLUT2) within the caudal/lateral LPN of the rat. For this zone, we found that all tracer‐labeled cortical terminals, as well as vGLUT1 antibody‐labeled terminals, are small profiles with round vesicles (RS profiles) that innervate small‐caliber dendrites. Tracer‐labeled tecto‐LPN terminals, as well as vGLUT2 antibody‐labeled terminals, were medium‐sized profiles with round vesicles (RM profiles). Tecto‐LPN terminals were significantly larger than cortico‐LPN terminals and contacted significantly larger dendrites. These results indicate that, within the tectorecipient zone of the rat LPN, cortical terminals are located distal to tectal terminals and that vGLUT1 and vGLUT2 antibodies may be used as markers for cortical and tectal terminals, respectively. Finally, comparisons of the synaptic patterns formed by tracer‐labeled terminals with those of vGLUT antibody‐labeled terminals suggest that individual LPN neurons receive input from multiple cortical and tectal axons. We suggest that the tectorecipient LPN constitutes a third category of thalamic nucleus (“second‐order”) that integrates convergent tectal and cortical inputs. This organization may function to signal the movement of novel or threatening objects moving across the visual field. J. Comp. Neurol. 515:647–663, 2009. © 2009 Wiley‐Liss, Inc.