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Expression and localization of the prion protein PrP C in the olfactory system of the mouse
Author(s) -
Le Pichon Claire E.,
Firestein Stuart
Publication year - 2008
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.21698
Subject(s) - olfactory bulb , biology , olfactory system , in situ hybridization , olfactory ensheathing glia , olfactory epithelium , neuroscience , immunohistochemistry , sensory system , olfactory marker protein , central nervous system , microbiology and biotechnology , nervous system , function (biology) , knockout mouse , gene expression , receptor , immunology , gene , genetics
Abstract The normal physiological function of the prion protein PrP C remains elusive despite its widespread expression, particularly throughout the nervous system. A critical step toward identifying its function is to precisely localize its pattern of expression. Historically, the immunolocalization of PrP C has proved to be notoriously difficult and nonconsensual. We have thus undertaken a detailed expression analysis by means of a combination of in situ hybridization, knockout mice, and immunohistochemistry, using recently generated highly specific antibodies. We have attempted to accurately localize PrP C expression in a tissue that is highly structured and of crucial behavioral importance to mice, the olfactory system. We found that PrP C was expressed in both peripheral and central neurons of the olfactory system and that its distribution was axonal‐specific in both olfactory sensory neurons of the olfactory epithelium and mitral cells of the olfactory bulb. Our detailed expression analysis and the axonal localization we observed may provide important hints toward potential functions of PrP C . J. Comp. Neurol. 508:487–499, 2008. © 2008 Wiley‐Liss, Inc.