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Patterns of laminins and integrins in the embryonic ventricular zone of the CNS
Author(s) -
Lathia Justin D.,
Patton Bruce,
Eckley D. Mark,
Magnus Tim,
Mughal Mohamed R.,
Sasaki Takako,
Caldwell Maeve A.,
Rao Mahendra S.,
Mattson Mark P.,
ffrenchConstant Charles
Publication year - 2007
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.21520
Subject(s) - biology , integrin , laminin , extracellular matrix , microbiology and biotechnology , neural stem cell , embryonic stem cell , neural development , neuroscience , stem cell , receptor , genetics , gene
The extracellular matrix (ECM) provides both a physical framework and a microenvironment that supplies instructive signals from the earliest stages of multicellular development. As a first step toward understanding the role of the ECM in regulating the behavior of neural stem cells (NSCs), here we show the localization of laminins, a heterotrimeric family of ECM molecules expressed in many different stem cell microenvironments, and their corresponding receptors in the embryonic murine ventricular zone (VZ) within which the NSCs undergo symmetrical and asymmetrical divisions required for cortical development. In addition to the presence of laminins containing both the α2 and α4 chains, we find distinct patterns of ECM receptor expression in the VZ and in the overlying cortex. Neural stem cells derived from the VZ express high levels of the integrin laminin receptor α6β1. At developmental stages at which NSCs undergo asymmetrical divisions, integrin β1 was unevenly distributed in some mitotic pairs at the ventricular wall. These results suggest a significant role in the regulation of NSC fate for laminin/integrin signaling within the microenvironment of the VZ and provide a framework for future molecular and cellular analyses of the role of the ECM in neural development. J. Comp. Neurol. 505:630–643, 2007. © 2007 Wiley‐Liss, Inc.