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Patterns of SDF‐1α and SDF‐1γ mRNAs, migration pathways, and phenotypes of CXCR4‐expressing neurons in the developing rat telencephalon
Author(s) -
Stumm Ralf,
Kolodziej Angela,
Schulz Stefan,
Kohtz Jhumku D.,
Höllt Volker
Publication year - 2007
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.21336
Subject(s) - calretinin , biology , ganglionic eminence , cerebrum , gabaergic , neuroscience , subventricular zone , neocortex , forebrain , subplate , cerebral cortex , anatomy , microbiology and biotechnology , neural stem cell , central nervous system , inhibitory postsynaptic potential , stem cell , immunohistochemistry , immunology
Cortical GABAergic neurons originate in the ventral telencephalon, invade the cortex via tangential migration, and integrate into the cortical plate by surface‐directed and ventricle‐directed migration. In mice lacking CXCR4 or SDF‐1, GABAergic neurons fail to complete their migration. It is presently unknown which parts of the migration of CXCR4‐expressing GABAergic neurons are driven by SDF‐1. Here we compared patterns of SDF‐1 isoforms and CXCR4 in the developing rat telencephalon. In the ventral telencephalon, radial glia, striatal, and migratory GABAergic neurons expressed CXCR4. Tangentially migrating CXCR4‐expressing neurons populated the marginal zone and started to invade the lateral intermediate zone at embryonic day (E)14. Until E17 the spread of CXCR4‐expressing neurons in the dorsomedial direction was accompanied by progressive upregulation of SDF‐1α in the dorsomedial intermediate/subventricular zone. In the meninges, SDF‐1α and SDF‐1γ were expressed persistently. During invasion of the cortical plate the orientation of CXCR4‐immunoreactive neurons changed gradually from tangential (E17/E18) to radial (postnatal day [P] 0), which was paralleled by downregulation of SDF‐1α in the intermediate/subventricular zone. At E17, CXCR4‐immunoreactive cells were colabeled with markers for ventral forebrain‐derived neurons (Dlx) but not markers for glutamatergic (Tbr) or subplate (calretinin) neurons. Postnatally, calretinin‐ and somatostatin‐expressing but not parvalbumin‐expressing GABAergic neurons or pyramidal cells contained CXCR4. Pyramidal cells and few large blood vessels expressed SDF‐1α, while microvessels contained SDF‐1γ transcripts. In summary, SDF‐1α is expressed along cortical but not subcortical migration routes of GABAergic neurons. We propose that regulated expression of SDF‐1 in the intermediate/subventricular zone influences lateromedial tangential migration of CXCR4‐expressing GABAergic neurons. J. Comp. Neurol. 502:382–399, 2007. © 2007 Wiley‐Liss, Inc.