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Nonintegrin laminin receptor precursor protein is expressed on olfactory stem and progenitor cells
Author(s) -
Jang Woochan,
Kim Kwang Pyo,
Schwob James E.
Publication year - 2007
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.21328
Subject(s) - biology , olfactory epithelium , progenitor cell , cell sorting , laminin , population , microbiology and biotechnology , stem cell , olfactory mucosa , olfactory marker protein , vomeronasal organ , monoclonal antibody , immunology , receptor , antibody , biochemistry , olfactory system , extracellular matrix , flow cytometry , neuroscience , demography , sociology
Abstract The biochemical identification and immunocytochemical characterization of a cell surface antigen, expressed on globose basal cells (GBCs) of the rodent olfactory epithelium (OE), are described. The monoclonal antibody (MAb) GBC‐3 recognizes a surface protein, confirmed by both live cell staining and fluorescence‐activated cell sorting. Two‐dimensional SDS‐PAGE/Western blot followed by tandem mass spectrometry demonstrates that the cell surface GBC‐3 antigen is the 40 kDa laminin receptor precursor protein. The MAb GBC‐3 labels the vast majority of cells among the GBC population and does not stain either sustentacular cells or horizontal basal cells (HBCs) in the normal rat OE. After epithelial lesion by exposure to methyl bromide, the remaining cells, which are mostly GBCs, are heavily stained by GBC‐3, and colabeled with GBC‐3 and sustentacular cell or HBC markers. GBC‐3 will be a potentially useful tool for identifying and characterizing GBCs. J. Comp. Neurol. 502:367–381, 2007. © 2007 Wiley‐Liss, Inc.