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Development of N‐methyl‐D‐aspartate receptor subunits in avian auditory brainstem
Author(s) -
Tang YeZhong,
Carr Catherine E.
Publication year - 2007
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.21303
Subject(s) - nmda receptor , brainstem , biology , cochlear nucleus , neuroscience , in situ hybridization , protein subunit , nucleus , auditory system , glutamate receptor , excitatory postsynaptic potential , receptor , microbiology and biotechnology , messenger rna , inhibitory postsynaptic potential , biochemistry , gene
N‐methyl‐D‐aspartate (NMDA) receptor subunit‐specific probes were used to characterize developmental changes in the distribution of excitatory amino acid receptors in the chicken's auditory brainstem nuclei. Although NR1 subunit expression does not change greatly during the development of the cochlear nuclei in the chicken (Tang and Carr [2004] Hear. Res 191:79–89), there are significant developmental changes in NR2 subunit expression. We used in situ hybridization against NR1, NR2A, NR2B, NR2C, and NR2D to compare NR1 and NR2 expression during development. All five NMDA subunits were expressed in the auditory brainstem before embryonic day (E) 10, when electrical activity and synaptic responses appear in the nucleus magnocellularis (NM) and the nucleus laminaris (NL). At this time, the dominant form of the receptor appeared to contain NR1 and NR2B. NR2A appeared to replace NR2B by E14, a time that coincides with synaptic refinement and evoked auditory responses. NR2C did not change greatly during auditory development, whereas NR2D increased from E10 and remained at fairly high levels into adulthood. Thus changes in NMDA NR2 receptor subunits may contribute to the development of auditory brainstem responses in the chick. J. Comp. Neurol. 502:400–413, 2007. © 2007 Wiley‐Liss, Inc.