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Phylogenetic preservation of α 3 Na + ,K + ‐ATPase distribution in vertebrate peripheral nervous systems
Author(s) -
Romanovsky Dmitry,
Moseley Amy E.,
Mrak Robert E.,
Taylor Michael D.,
Dobretsov Maxim
Publication year - 2006
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.21218
Subject(s) - biology , gene isoform , peripheral nervous system , vertebrate , efferent , amphibian , central nervous system , nervous system , anatomy , microbiology and biotechnology , neuroscience , afferent , biochemistry , gene , ecology
The α 3 isoform of Na + ,K + ‐ATPase is uniquely expressed in afferent and efferent neurons innervating muscle spindles in the peripheral nervous system (PNS) of adult rats, but the distribution pattern of this isoform in other species has not been investigated. We compared expression of α 3 Na + ,K + ‐ATPase in lumbar dorsal root ganglia (DRG), spinal roots, and skeletal muscle samples of amphibian (frog), reptilian (turtle), avian (pigeon and chicken), and mammalian (mouse and human) species. In all species studied, the α 3 Na + ,K + ‐ATPase isoform was nonuniformly expressed in peripheral ganglia and nerves. In spinal ganglia, only 5–20% of neurons expressed this isoform, and, in avian and mammalian species, these α 3 Na + ,K + ‐ATPase‐expressing neurons belonged to a subpopulation of large DRG neurons. In ventral root fibers of pigeons, mice, and humans, the α 3 Na + ,K + ‐ATPase was abundantly expressed predominantly in small myelinated axons. In skeletal muscle samples from turtles, pigeons, mice, and humans, α 3 Na + ,K + ‐ATPase was detected in intramuscular myelinated axons and in profiles of nerve terminals associated with the equatorial and polar regions of muscle spindle intrafusal fibers. These results show that the expression profiles for α 3 Na + ,K + ‐ATPase in the peripheral nervous system of a wide variety of vertebrate species are similar to the profile of rats and suggest that stretch receptor‐associated expression of α 3 Na + ,K + ‐ATPase is preserved through vertebrate evolution. J. Comp. Neurol. 500:1106–1116, 2007. © 2006 Wiley‐Liss, Inc.