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Neurons and glial cells differentially express P2Y receptor mRNAs in the rat dorsal root ganglion and spinal cord
Author(s) -
Kobayashi Kimiko,
Fukuoka Tetsuo,
Yamanaka Hiroki,
Dai Yi,
Obata Koichi,
Tokunaga Atsushi,
Noguchi Koichi
Publication year - 2006
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.21066
Subject(s) - biology , spinal cord , dorsal root ganglion , in situ hybridization , neuroscience , gdf7 , p2y receptor , microbiology and biotechnology , receptor , ganglion , astrocyte , central nervous system , purinergic receptor , anatomy , messenger rna , embryonic stem cell , gene , biochemistry , extracellular
We examined the precise distribution of mRNAs for six cloned rat P2Y receptor subtypes, P2Y1, P2Y2, P2Y4, P2Y6, P2Y12, and P2Y14, in the dorsal root ganglion (DRG) and spinal cord by in situ hybridization histochemistry (ISHH) with 35 S‐labeled riboprobes. In the DRG, P2Y1 and P2Y2 mRNAs were expressed by 15% and 24% of all neurons, respectively. Although each receptor was evenly distributed between neurofilament‐positive and ‐negative neurons, P2Y2 was rather selectively expressed by TrkA‐positive neurons. Schwann cells expressed P2Y2 mRNA, and the nonneuronal cells around the DRG neurons, perhaps the satellite cells, expressed P2Y12 and P2Y14 mRNAs. No ISHH signals for P2Y4 or P2Y6 were seen in any cellular components of the DRG. In the spinal cord, P2Y1 and P2Y4 mRNAs were expressed by some of the dorsal horn neurons, whereas the motor neurons in the ventral horn had P2Y4 and P2Y6 mRNAs. In addition, astrocytes in the gray matter had P2Y1 mRNA, and the microglia throughout the spinal cord expressed P2Y12 mRNA. P2Y14 mRNA was weakly expressed by putative microglia. These findings should provide useful information in interpreting pharmacological and electrophysiological studies in this field given the lack of highly selective antagonists for each P2Y receptor subtype. J. Comp. Neurol. 498:443–454, 2006. © 2006 Wiley‐Liss, Inc.