z-logo
Premium
Localization and characterization of pituitary adenylate cyclase‐activating polypeptide receptors in the human cerebellum during development
Author(s) -
Basille Magali,
Cartier Dorthe,
Vaudry David,
Lihrmann Isabelle,
Fournier Alain,
Freger Pierre,
GalloPayet Nicole,
Vaudry Hubert,
Gonzalez Bruno
Publication year - 2006
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.20934
Subject(s) - cerebellum , granule cell , biology , in situ hybridization , receptor , pituitary adenylate cyclase activating peptide , messenger rna , population , medicine , endocrinology , cerebellar cortex , microbiology and biotechnology , neuropeptide , central nervous system , vasoactive intestinal peptide , biochemistry , gene , dentate gyrus , demography , sociology
Pituitary adenylate cyclase‐activating polypeptide (PACAP) receptors are actively expressed in the cortical layers of the cerebellum of rodents and contribute to cerebellar development. The present report provides the first anatomical localization and characterization of PACAP receptors in the developing human cerebellum. RT‐PCR analysis from 15‐week‐old fetuses to 22‐year‐old subject showed that PAC1‐R and VPAC1‐R are expressed in the cerebellum at all stages, whereas VPAC2‐R mRNA was barely detectable. In situ hybridization labeling indicated that, in human fetuses, PAC1‐R mRNA is associated with the external granule cell layer (EGL), a germinative neuroepithelium, and with the internal granule cell layer (IGL). The distribution pattern of VPAC1‐R mRNA was very similar to that of PAC1‐R mRNA, whereas VPAC2‐R mRNA was visualized only in 7–22‐year‐old subjects. The localization of [ 125 I]PACAP27 binding sites was fully consistent with the distribution of PAC1‐R and VPAC1‐R mRNA. Pharmacological characterization revealed that, in the EGL and IGL from 15–24‐week‐old fetuses and in the granule cell layer from 7–22‐year‐old patients, binding sites exhibit a PAC1‐R profile. In contrast, PACAP binding sites observed in the molecular layer and medulla of the adult cerebellum consisted of a heterogeneous population of PAC1‐R and VPAC(1/2)‐R. Altogether, these data provide the first evidence that PACAP receptors are expressed in the human cerebellar cortex. PAC1‐R is the predominant PACAP receptor found in fetuses, and both PAC1‐R and VPAC1‐R are expressed in the mature cerebellum. These observations suggest that PACAP has neurodevelopmental functions in the human cerebellum. J. Comp. Neurol. 496:468–478, 2006. © 2006 Wiley‐Liss, Inc.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here