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Complementary stripes of phospholipase Cβ3 and Cβ4 expression by Purkinje cell subsets in the mouse cerebellum
Author(s) -
Sarna Justyna R.,
Marzban Hassan,
Watanabe Masahiko,
Hawkes Richard
Publication year - 2006
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.20912
Subject(s) - biology , purkinje cell , cerebellum , neuroscience , cerebellar cortex , microbiology and biotechnology , parallel fiber , phospholipase c , phospholipase , signal transduction , biochemistry , enzyme
Transverse boundaries divide the cerebellar cortex into four transverse zones, and within each zone the cortex is further subdivided into a symmetrical array of parasagittal stripes. Several molecules believed to mediate long‐term depression at the parallel fiber–Purkinje cell synapse are known to be expressed in stripes. We have therefore explored the distributions of phospholipase Cβ3 and phospholipase Cβ4, key components in the transduction of type 1 metabotropic glutamate receptor‐mediated responses. The data reveal that both phospholipase Cβ isotypes are expressed strongly in the mouse cerebellum in subsets of Purkinje cells. The two distributions are distinct and largely nonoverlapping. The pattern of phospholipase Cβ3 expression is unique, revealing stripes in three of the four transverse zones and a uniform distribution in the fourth. In contrast, phospholipase Cβ4 appears to be confined largely to the Purkinje cells that are phospholipase Cβ3‐negative. PLCβ3 is restricted to the zebrin II‐immunopositive Purkinje cell subset. Not all zebrin II‐immunoreactive Purkinje cells express PLCβ3: in lobules IX and X it is restricted to that zebrin II‐immunopositive subset that also expresses the small heat shock protein HSP25. PLCβ4 expression is restricted to, and coextensive with, the zebrin II‐immunonegative Purkinje cell subset. These nonoverlapping expression patterns suggest that long‐term depression may be manifested differently between cerebellar modules. J. Comp. Neurol. 496:303–313, 2006. © 2006 Wiley‐Liss, Inc.