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Localization of 5α‐reductase in the rat main olfactory bulb
Author(s) -
Kiyokage Emi,
Toida Kazunori,
SuzukiYamamoto Toshiko,
Ishimura Kazunori
Publication year - 2005
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.20760
Subject(s) - olfactory bulb , olfactory ensheathing glia , biology , olfactory system , olfactory nerve , immunocytochemistry , immunolabeling , reductase , neuroglia , biochemistry , microbiology and biotechnology , medicine , endocrinology , central nervous system , immunohistochemistry , neuroscience , enzyme , immunology
The enzyme steroid 5α‐reductase catalyzes the production of dihydroprogesterone and dihydrotestosterone, which were recently recognized as neurosteroids in the brain with variably potential neuroactivity. The present study reports for the first time detailed localization of 5α‐reductase type 1 in the rat main olfactory bulb. The occurrence of 5α‐reductase in the olfactory bulb was detected by reverse transcription‐polymerase chain reaction and Western blotting analyses. In addition, the enzyme activity was also detected by thin layer chromatography. Immunocytochemistry showed that 5α‐reductase immunoreactive cells of variable intensity were present in all layers of the olfactory bulb. Multiple immunolabeling revealed that 5α‐reductase was mainly localized in glial cells, namely, in S‐100β‐ and glial fibrillary acidic protein‐immunoreactive astrocytes, 2′, 3′‐cyclic nucleotide 3′‐phosphodiesterase (CNPase)‐immunoreactive oligodendrocytes, and in S‐100β‐ and neuropeptide‐Y‐immunoreactive olfactory ensheathing cells, whereas the bulbar neurons exhibited little immunoreactivity. Quantitative analysis revealed that the number of 5α‐reductase‐immunoreactive cells was greatest in the olfactory nerve layer. The most intense 5α‐reductase‐immunoreactivity was found in the olfactory ensheathing cells, and next in the CNPase‐immunoreactive cells. The 5α‐reductase in the olfactory bulb was expressed constantly throughout different ages and sexes and in neutered and hypophysectomized rats. Thus, 5α‐reductase may contribute via 5α‐reduced metabolites to the formation and maintenance of olfactory inputs and outputs, which were closely associated with the olfactory ensheathing cells and the oligodendrocytes, respectively. J. Comp. Neurol. 493:381–395, 2005. © 2005 Wiley‐Liss, Inc.