Premium
Molecular and genetic features of a labeled class of spinal substantia gelatinosa neurons in a transgenic mouse
Author(s) -
Hantman Adam W.,
Perl Edward R.
Publication year - 2005
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.20709
Subject(s) - biology , green fluorescent protein , transgene , microbiology and biotechnology , tyrosine hydroxylase , genetically modified mouse , gene , genetics , biochemistry , enzyme
Genetic incorporation in a mouse of a transgene containing the prion promoter and the green fluorescent protein (GFP) coding sequence labels a set of substantia gelatinosa (SG) neurons (SG‐GFP) homogenous in morphology, electrophysiology, and γ‐amino‐butyric acid expression. In the present analysis the SG‐GFP neurons are established to have protein kinase C‐βII immunoreactivity and to lack evidence for the presence of calbindin D‐28k, parvalbumin, and protein kinase C‐γ. These neurons were hyperpolarized by mediators of descending control, norepinephrine and serotonin. Sequential polymerase chain reactions established the insertion of the transgene to be in the receptor protein tyrosine phosphatase kappa (RPTP‐κ) and the laminin receptor 1 (ribosomal protein SA) pseudogene 1 locus. RPTP‐κ expression in both GFP‐labeled dorsal root ganglia and SG neurons raises the possibility that homophilic interactions of RPTP‐κ contribute to establishment of connections between specific classes of primary afferent and SG neurons. J. Comp. Neurol. 492:90–100, 2005. © 2005 Wiley‐Liss, Inc.