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Differential development of 5‐HT receptor and the serotonin transporter binding in the human infant medulla
Author(s) -
Paterson David S.,
Belliveau Richard A.,
Trachtenberg Felicia,
Kinney Hannah C.
Publication year - 2004
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.20105
Subject(s) - biology , 5 ht receptor , endocrinology , serotonin , medicine , receptor , hypoglossal nucleus , serotonin transporter , binding potential , raphe nuclei , 5 ht1a receptor , central nervous system , serotonergic , biochemistry
Tissue receptor autoradiography with 3 H‐lysergic acid diethylamide ( 3 H‐LSD), 3 H‐8‐hydroxy‐2‐[di‐N‐propylamine] tetralin ( 3 H‐8‐OH‐DPAT), and 125 I‐RTI‐55 was used to map the distribution and developmental profile of 5‐HT 1A–1D and 5‐HT 2 receptors, 5‐HT 1A receptors, and the serotonin (5‐HT) transporter (SERT), respectively, to nuclei with cardiorespiratory function in the human medulla from midgestation to maturity. The distribution pattern of the 5‐HT markers was heterogeneous, with variable densities of binding of each observed both in nuclei with and without 5‐HT cell bodies. The highest density of binding for each marker was observed in the raphé nuclei, the site of the highest density of 5‐HT cell bodies. A significant reduction in 5‐HT receptor binding measured with 3 H‐LSD was observed between midgestation and infancy, and between infancy and maturity in multiple nuclei, but no changes were observed across infancy. A significant increase in 5‐HT 1A receptor binding density was observed across infancy in the hypoglossal nucleus (regression slope coefficient = 0.008 ± 0.002, P = 0.02), and a marginally significant increase was observed in the raphé obscurus (regression slope coefficient = 0.061 ± 0.026 [mean ± SEM], P = 0.05). No significant age‐related changes in SERT binding were observed at any time. With the exception of the hypoglossal nucleus, where 5‐HT 1A receptor binding increases while SERT binding remains stable, the medullary 5‐HT markers analyzed in the study are essentially “in place” at birth. This study provides important baseline data that serve as a foundation for future work in pediatric 5‐HT brainstem disorders, including sudden infant death syndrome. J. Comp. Neurol. 472:221–231, 2004. © 2004 Wiley‐Liss, Inc.