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Synaptic patterns of thalamocortical afferents in mouse barrels at postnatal day 11
Author(s) -
Lev Dmitri L.,
Weinfeld Elizabeth,
White Edward L.
Publication year - 2001
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.1422
Subject(s) - biotinylated dextran amine , synaptogenesis , neuroscience , biology , thalamus , synapse , dendritic spine , barrel cortex , axon , anatomy , somatosensory system , hippocampal formation
Abstract This study focuses on the synaptic output patterns of thalamocortical axons in mouse barrel cortex at postnatal day (P) 11. Axons were labeled by biotinylated dextran amine transported anterogradely following injection in vivo into the ventrobasal thalamus. Labeled axons in the posteromedial barrel subfield were examined by light and electron microscopy and then reconstructed in three dimensions to assess the spatial distribution of their synapses. Thalamocortical axons form asymmetrical synapses, both at varicosities and along cylindrical portions of the axons; usually, only one synapse occurs per site, contrasting with the case in the adult, in which multiple synapses are typical. At P11, varicosities without synapses are common. As in adult barrels, approximately 80% of synapses formed by thalamocortical axons are with dendritic spines; 20% are with dendritic shafts. The similarity in the distribution of thalamocortical synapses onto spines vs. dendrites in developing and mature barrels indicates that adult synaptic patterns already are specified at a very early stage of thalamocortical synaptogenesis. J. Comp. Neurol. 442:63–77, 2002. ©2002 Wiley‐Liss, Inc.