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Isoform‐specific distribution of the plasma membrane Ca 2+ ATPase in the rat brain
Author(s) -
Burette Alain,
Rockwood Julia M.,
Strehler Emanuel E.,
Weinberg Richard J.
Publication year - 2003
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.10933
Subject(s) - plasma membrane ca2+ atpase , biology , gene isoform , cytoplasm , calcium pump , microbiology and biotechnology , extracellular , calcium , calcium atpase , homeostasis , subcellular localization , atpase , intracellular , calcium binding protein , biochemistry , medicine , enzyme , gene
Regulation of cytoplasmic calcium is crucial both for proper neuronal function and cell survival. The concentration of Ca 2+ in cytoplasm of a neuron at rest is 10,000 times lower than in the extracellular space, pointing to the importance of the transporters that extrude intracellular Ca 2+ . The family of plasma membrane calcium‐dependent ATPases (PMCAs) represent a major component of the Ca 2+ regulatory system. However, little information is available on the regional and cellular distribution of these calcium pumps. We used immunohistochemistry to investigate the distribution of each of the four PMCA isoforms (PMCA1–4) in the rat brain. Each isoform exhibited a remarkably precise and distinct pattern of distribution. In many cases, PMCA isoforms in a single brain structure were differentially expressed within different classes of neurons, and within different subcellular compartments. These data show that each isoform is independently organized and suggest that PMCAs may play a more complex role in calcium homeostasis than generally recognized. J. Comp. Neurol. 467:464–476, 2003. © 2003 Wiley‐Liss, Inc.