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Developmental changes in expression patterns of two dopamine receptor genes in mushroom bodies of the honeybee, Apis mellifera
Author(s) -
Kurshan Peri T.,
Hamilton Ingrid S.,
Mustard Julie A.,
Mercer Alison R.
Publication year - 2003
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.10864
Subject(s) - mushroom bodies , biology , dopamine , microbiology and biotechnology , gene expression , receptor , dopamine receptor , mushroom , gene , neuroscience , botany , genetics , drosophila melanogaster
The expression patterns of two dopamine receptor genes, Am dop1 and Am dop2 , in the developing mushroom bodies of the honeybee brain were determined by using in situ hybridisation. Both genes were expressed throughout pupal development, but their patterns of expression in the three major divisions of mushroom body intrinsic neurons (outer compact cells, noncompact cells, and inner compact cells) were quite distinct. Am dop1 expression could be detected in all three mushroom body cell groups throughout development. Staining for Am dop1 mRNA was particularly intense in newly born Kenyon cells, suggesting that levels of Am dop1 expression are higher in newborn cells than in more mature mushroom body neurons. This was not the case for Am dop2 . Am dop2 expression in the mushroom bodies was restricted to inner and outer compact cells during most of pupal development, appearing in noncompact cells only late in metamorphosis or at adult eclosion. In contrast to the case with Am dop1 , staining for Am dop2 mRNA was observed in glial cells. Expression of Am dop2 in glial cells was detected only at early stages of glial cell development, when the cells are reported to be actively dividing. This study not only implicates dopamine in the development of honeybee mushroom bodies but also suggests different roles for the two dopamine receptors investigated. J. Comp. Neurol. 466:91–103, 2003. © 2003 Wiley‐Liss, Inc.