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Ectopic corticospinal tract and corticothalamic tract neurons in the cerebral cortex of yotari and reeler mice
Author(s) -
Yamamoto Tatsuro,
Sakakibara Shunsuke,
Mikoshiba Katsuhiko,
Terashima Toshio
Publication year - 2003
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.10678
Subject(s) - reeler , reelin , cerebral cortex , biology , neuroscience , cortex (anatomy) , horseradish peroxidase , thalamus , neocortex , anatomy , corticospinal tract , wheat germ agglutinin , dab1 , microbiology and biotechnology , medicine , magnetic resonance imaging , lectin , biochemistry , radiology , diffusion mri , extracellular matrix , enzyme
Reeler and yotari mice, which are mutant for Reelin or Dab1, respectively, show disorders of cerebral cortical lamination. We injected horseradish peroxidase (HRP) into the upper lumbar enlargement to label corticospinal tract (CST) neurons and wheat germ agglutinin‐conjugated HRP (WGA‐HRP) into the ventral lateral nucleus of the thalamus to label corticothalamic tract (CTT) neurons in both 19‐day‐old yotari and reeler mice with the aim of discovering whether or not they show differences in the distribution pattern of layer V or layer VI neurons. Similar injections of tracers were made in normal controls. HRP‐labeled CST neurons, which were exclusively distributed in layer V of the normal cortex, were radially scattered in the cortex of both mutants, but those in reeler were more deeply distributed than in yotari . WGA‐labeled CTT neurons, which were mainly located in layer VI in the normal cortex, were superficially distributed just beneath the pia mater in both reeler and yotari cortex. The present quantitative study shows that the distribution pattern of layer V neurons, but not layer VI neurons, differs between reeler and yotari mice, suggesting that the Reelin and Dab1 proteins may play different roles in the migration and cell positioning of layer V neurons. J. Comp. Neurol. 461:61–75, 2003. © 2003 Wiley‐Liss, Inc.