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Spatiotemporal distribution of neuronal calcium sensor‐1 in the developing rat spinal cord
Author(s) -
Kawasaki Takayuki,
Nishio Takeshi,
Kurosawa Hisashi,
Roder John,
Jeromin Andreas
Publication year - 2003
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.10649
Subject(s) - synaptophysin , neuropil , spinal cord , biology , synaptogenesis , white matter , glial fibrillary acidic protein , astrocyte , calretinin , neuroscience , anatomy , central nervous system , immunohistochemistry , immunology , magnetic resonance imaging , medicine , radiology
The present study revealed the localization of neuronal calcium sensor (NCS)‐1 immunoreactivity (IR) in the developing rat spinal cord. The NCS‐1 IR first appeared at embryonic day 12 in the peripheral nerves and their somata. Intense NCS‐1 IR was expressed in ascending and descending tracts in the white matter during the late prenatal period, which gradually decreased to the faint level during postnatal development. Intense NCS‐1 IR was colocalized with growth associated protein (GAP)‐43 IR in the marginal zone and with the glutamate‐aspartate transporter (GLAST) IR in the radial processes traversing the marginal zone. In the adult rat white matter, radially oriented astrocytes and astrocytes in the glia limitans were double‐labeled for NCS‐1 and glial fibrillary acidic protein (GFAP), whereas small dots on finger‐like dendritic projections were double‐labeled for NCS‐1 and synaptophysin. In the developing gray matter, the NCS‐1 IR appeared at embryonic day 12 and gradually increased in the neuronal somata and neuropil, reaching a plateau after the end of the 4th postnatal week. The small dots in neuropil were colabeled for NCS‐1 and GFAP or NCS‐1 and synaptophysin in the adult rat gray matter. These results strongly suggest that NCS‐1 is involved in axogenesis and synaptogenesis in the developing rat spinal cord. NCS‐1 can serve as a Ca 2+ ‐sensor not only in neurons but also in radial glial cells or even in radially oriented astrocytes in the adult rat spinal cord. J. Comp. Neurol. 460:465–475, 2003. © 2003 Wiley‐Liss, Inc.